PrimePCR™ Probe Assay: VIPR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VIPR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VIPR2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031657
Assay Design:   Intron-spanning
Chromosome Location:   7:158824651-158826939question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000262178 ENST00000377633 ENST00000402066

Gene Information

This gene encodes a receptor for vasoactive intestinal peptide a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation exocrine and endocrine secretion and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. [provided by RefSeq Aug 2011]

Gene Symbol:   VIPR2
Gene Name:   vasoactive intestinal peptide receptor 2
Aliases:   FLJ16511, VPAC2, VPCAP2R
RefSeq:   NC_000007.13 NT_007741.14
Ensembl:   ENSG00000106018
Entrez:   7434
Chromosome Mapping:   7q36.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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