PrimePCR™ Probe Assay: PFKFB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PFKFB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PFKFB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031766
Assay Design:   Intron-spanning
Chromosome Location:   1:207241559-207242826question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000367080 ENST00000367079 ENST00000545806 ENST00000411990 ENST00000541914

Gene Information

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-26-bisphosphate a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-26-bisphosphate and a fructose-26-biphosphatase activity that catalyzes the degradation of fructose-26-bisphosphate. This protein regulates fructose-26-bisphosphate levels in the heart while a related enzyme encoded by a different gene regulates fructose-26-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PFKFB2
Gene Name:   6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Aliases:   DKFZp781D2217, MGC138308, MGC138310, PFK-2/FBPase-2
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000123836
Entrez:   5208
Chromosome Mapping:   1q31

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