PrimePCR™ Probe Assay: FMO4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FMO4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FMO4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031869
Assay Design:   Intron-spanning
Chromosome Location:   1:171283501-171285418question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000367749

Gene Information

Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme FMO1 found in fetal liver FMO2 found in adult liver and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs pesticides and xenobiotics. [provided by RefSeq Jul 2008]

Gene Symbol:   FMO4
Gene Name:   flavin containing monooxygenase 4
Aliases:   FMO2
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000076258
Entrez:   2329
Chromosome Mapping:   1q24.3

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