PrimePCR™ Probe Assay: GPR116, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032834
Assay Design:   Intron-spanning
Chromosome Location:   6:46827187-46828511question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000265417 ENST00000452370 ENST00000283296 ENST00000362015 ENST00000456426 ENST00000545557 ENST00000545669

Gene Information

Description Not Available

Gene Symbol:   GPR116
Gene Name:   G protein-coupled receptor 116
Aliases:   DKFZp564O1923, FLJ90640, KIAA0758, KPG_001
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000069122
Entrez:   221395
Chromosome Mapping:   6p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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