PrimePCR™ Probe Assay: SMG6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SMG6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SMG6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032837
Assay Design:   Intron-spanning
Chromosome Location:   17:1985178-1989047question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000263073 ENST00000544865 ENST00000536871 ENST00000354901

Gene Information

This gene encodes a protein which is part of the telomerase ribonucleoprotein complex and binds single-stranded DNA found at the telomeres but the product of this gene also participates in the nonsense-mediated mRNA decay (NMD) pathway. The encoded protein contains two binding sites for components of the exon junction complex (EJC); interaction between the encoded protein and the EJC is necessary for NMD activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2012]

Gene Symbol:   SMG6
Gene Name:   Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Aliases:   C17orf31, EST1A, KIAA0732, SMG-6
RefSeq:   NG_027689.1 NC_000017.10 NT_010718.16
Ensembl:   ENSG00000070366
Entrez:   23293
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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