PrimePCR™ Probe Assay: BUB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033109
Assay Design:   Intron-spanning
Chromosome Location:   2:111419314-111423902question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000409311 ENST00000302759 ENST00000535254 ENST00000541432

Gene Information

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   BUB1
Gene Name:   budding uninhibited by benzimidazoles 1 homolog (yeast)
Aliases:   BUB1A, BUB1L, hBUB1
RefSeq:   NC_000002.11 NG_012048.1 NT_022135.16
Ensembl:   ENSG00000169679
Entrez:   699
Chromosome Mapping:   2q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.130000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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