PrimePCR™ Probe Assay: BUB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BUB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BUB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033109
Assay Design:   Intron-spanning
Chromosome Location:   2:111419314-111423902question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000409311 ENST00000302759 ENST00000535254 ENST00000541432

Gene Information

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq Jul 2008]

Gene Symbol:   BUB1
Gene Name:   budding uninhibited by benzimidazoles 1 homolog (yeast)
Aliases:   BUB1A, BUB1L, hBUB1
RefSeq:   NC_000002.11 NG_012048.1 NT_022135.16
Ensembl:   ENSG00000169679
Entrez:   699
Chromosome Mapping:   2q14

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