PrimePCR™ Probe Assay: HDAC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HDAC5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HDAC5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033250
Assay Design:   Intron-spanning
Chromosome Location:   17:42158153-42160015question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000225983 ENST00000393622 ENST00000336057

Gene Information

Histones play a critical role in transcriptional regulation cell cycle progression and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   HDAC5
Gene Name:   histone deacetylase 5
Aliases:   FLJ90614, HD5, NY-CO-9
RefSeq:   NC_000017.10 NG_015818.1 NT_010783.15
Ensembl:   ENSG00000108840
Entrez:   10014
Chromosome Mapping:   17q21

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