PrimePCR™ Probe Assay: TRDMT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TRDMT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRDMT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033711
Assay Design:   Intron-spanning
Chromosome Location:   10:17195617-17199516question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000377799 ENST00000488990 ENST00000412821 ENST00000351358 ENST00000457442 ENST00000377766 ENST00000358282

Gene Information

This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq Jun 2010]

Gene Symbol:   TRDMT1
Gene Name:   tRNA aspartic acid methyltransferase 1
Aliases:   DMNT2, DNMT2, MHSAIIP, PUMET, RNMT1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000107614
Entrez:   1787
Chromosome Mapping:   10p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.480000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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