PrimePCR™ Probe Assay: USH2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: USH2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: USH2A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039032
Assay Design:   Intron-spanning
Chromosome Location:   1:216390850-216405368question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000307340 ENST00000366942 ENST00000366943

Gene Information

This gene encodes a protein that contains laminin EGF motifs a pentaxin domain and many fibronectin type III motifs. The protein is found in the basement membrane and may be important in development and homeostasis of the inner ear and retina. Mutations within this gene have been associated with Usher syndrome type IIa and retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   USH2A
Gene Name:   Usher syndrome 2A (autosomal recessive, mild)
Aliases:   RP39, US2, USH2, dJ1111A8.1
RefSeq:   NC_000001.10 NT_167186.1 NG_009497.1
Ensembl:   ENSG00000042781
Entrez:   7399
Chromosome Mapping:   1q41

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