PrimePCR™ Probe Assay: PARD3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PARD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PARD3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039350
Assay Design:   Intron-spanning
Chromosome Location:   10:34636959-34648111question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000374789 ENST00000374788 ENST00000346874 ENST00000374794 ENST00000350537 ENST00000374790 ENST00000374776 ENST00000374773 ENST00000374768 ENST00000545693 ENST00000545260 ENST00000340077 ENST00000544292

Gene Information

This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   PARD3
Gene Name:   par-3 partitioning defective 3 homolog (C. elegans)
Aliases:   ASIP, Baz, Bazooka, FLJ21015, PAR3, PAR3alpha, PARD3A, SE2-5L16, SE2-5LT1, SE2-5T2
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000148498
Entrez:   56288
Chromosome Mapping:   10p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 36.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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