PrimePCR™ Probe Assay: CSNK1G3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CSNK1G3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CSNK1G3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039360
Assay Design:   Intron-spanning
Chromosome Location:   5:122926198-122927104question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000511130 ENST00000512718 ENST00000521364 ENST00000510842 ENST00000361991 ENST00000360683 ENST00000515322 ENST00000395412 ENST00000395411 ENST00000345990

Gene Information

Description Not Available

Gene Symbol:   CSNK1G3
Gene Name:   casein kinase 1, gamma 3
Aliases:   Not Available
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000151292
Entrez:   1456
Chromosome Mapping:   5q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.080000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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