PrimePCR™ Probe Assay: APOBEC3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: APOBEC3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: APOBEC3B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039581
Assay Design:   Intron-spanning
Chromosome Location:   22:39382348-39385505question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000333467 ENST00000402182 ENST00000407298

Gene Information

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster thought to result from gene duplication on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A commonly-found deletion polymorphism of approximately 29.5 kb removes the 3' UTR of the adjacent APOBEC3A gene and the coding region of this gene and creates a hybrid of the two genes. [provided by RefSeq Jul 2011]

Gene Symbol:   APOBEC3B
Gene Name:   apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
Aliases:   APOBEC1L, ARCD3, ARP4, DJ742C19.2, FLJ21201, PHRBNL, bK150C2.2
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000179750
Entrez:   9582
Chromosome Mapping:   22q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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