PrimePCR™ Probe Assay: DEFB4B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: DEFB4B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DEFB4B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039891
Assay Design:   Intron-spanning
Chromosome Location:   8:7272465-7274355question
Amplicon Length:   189
Splice Variants Targeted:   ENST00000537330 ENST00000306801 ENST00000570891 ENST00000322652 ENST00000225394 ENST00000394869 ENST00000581348 ENST00000579937 ENST00000265605 ENST00000367845 ENST00000367847 ENST00000447632 ENST00000371747 ENST00000262676 ENST00000262873 ENST00000302247 ENST00000435817 ENST00000522126 ENST00000522783 ENST00000403714 ENST00000402191 ENST00000273317 ENST00000380456 ENST00000393423 ENST00000296137 ENST00000535325 ENST00000296849 ENST00000357447 ENST00000378503 ENST00000358621 ENST00000257189 ENST

Gene Information

Description Not Available

Gene Symbol:   DEFB4B
Gene Name:   defensin, beta 4B
Aliases:   DEFB4P
RefSeq:   NC_000008.10 NT_023736.17
Ensembl:   ENSG00000177257
Entrez:   100289462
Chromosome Mapping:   8p23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.370000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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