PrimePCR™ Probe Assay: HLA-DRB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: HLA-DRB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DRB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039968
Assay Design:   Intron-spanning
Chromosome Location:   6:32546835-32548601question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000334122 ENST00000564373 ENST00000569393 ENST00000392975 ENST00000360793 ENST00000584031 ENST00000581573 ENST00000448884 ENST00000582034 ENST00000542042 ENST00000580691 ENST00000582997 ENST00000584291 ENST00000392976 ENST00000576599 ENST00000320865 ENST00000444645 ENST00000457451 ENST00000334000 ENST00000397835 ENST00000414786 ENST00000411959 ENST00000437784 ENST00000465829 ENST00000481893 ENST00000306682 ENST00000541662 ENST00000340291 ENST00000262940 ENST00000461209 ENST00000449970 ENST

Gene Information

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB) both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3 DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3 DRB4 and DRB5. There are 4 related pseudogenes: DRB2 DRB6 DRB7 DRB8 and DRB9. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DRB1
Gene Name:   major histocompatibility complex, class II, DR beta 1
Aliases:   DRB1, DRw10, FLJ75017, FLJ76359, HLA-DR1B, HLA-DRB, HLA-DRB1*, SS1
RefSeq:   NC_000006.11 NG_002392.2 NG_002432.1 NG_002433.1 NT_007592.15 NT_113891.2 NT_167249.1
Ensembl:   ENSG00000196126
Entrez:   3123
Chromosome Mapping:   6p21.3

[ Close ]
Click here to print
[ Close ]
Click here to print
 

undefined

undefined

My PrimePCR Hot List stores your saved PrimePCR products and configurations

My PrimePCR Hot List stores your saved PrimePCR products and configurations

undefined

undefined

undefined

undefined

undefined

undefined

undefined