PrimePCR™ Probe Assay: TRIM10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TRIM10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRIM10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040074
Assay Design:   Intron-spanning
Chromosome Location:   6:30124758-30126228question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000553004 ENST00000448645 ENST00000428611 ENST00000374429 ENST00000287585 ENST00000449208 ENST00000446013 ENST00000553167 ENST00000426996 ENST00000413055 ENST00000551474 ENST00000456988 ENST00000416714 ENST00000546716 ENST00000449742 ENST00000376704 ENST00000368013 ENST00000368015 ENST00000243253 ENST00000424880 ENST00000417411 ENST00000451727 ENST00000548891 ENST00000354038 ENST00000259941 ENST00000548533 ENST00000431258 ENST00000447465 ENST00000551591 ENST00000398665 ENST00000440640 ENST

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. This protein localizes to cytoplasmic bodies. Studies in mice suggest that this protein plays a role in terminal differentiation of erythroid cells. Alternate splicing of this gene generates two transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   TRIM10
Gene Name:   tripartite motif containing 10
Aliases:   HERF1, MGC141979, RFB30, RNF9
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204613
Entrez:   10107
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996300
y-intercept 37.180000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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