PrimePCR™ Probe Assay: TRIM27, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TRIM27, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRIM27, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040090
Assay Design:   Intron-spanning
Chromosome Location:   6:28889695-28891025question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000523826 ENST00000339803 ENST00000379808 ENST00000552788 ENST00000549814 ENST00000524140 ENST00000400720 ENST00000417660 ENST00000437160 ENST00000412687 ENST00000437415 ENST00000436621 ENST00000327987 ENST00000558354 ENST00000431123 ENST00000452265 ENST00000455861 ENST00000430891 ENST00000435528 ENST00000427689 ENST00000377194 ENST00000377199

Gene Information

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq Jul 2008]

Gene Symbol:   TRIM27
Gene Name:   tripartite motif containing 27
Aliases:   RFP, RNF76
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204713
Entrez:   5987
Chromosome Mapping:   6p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.580000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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