PrimePCR™ Probe Assay: SSX2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SSX2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SSX2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040219
Assay Design:   Intron-spanning
Chromosome Location:   X:52729610-52734239question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000244533 ENST00000372530 ENST00000298181 ENST00000336777 ENST00000337502 ENST00000375515 ENST00000276049 ENST00000409816 ENST00000389524 ENST00000428314 ENST00000360890 ENST00000325239 ENST00000413659 ENST00000376932 ENST00000319275 ENST00000376923 ENST00000311798 ENST00000347757 ENST00000376919 ENST00000376909 ENST00000407081 ENST00000298396 ENST00000376893 ENST00000359376 ENST00000308511 ENST00000389279 ENST00000520861 ENST00000520738 ENST00000289388 ENST00000355728 ENST00000594102 ENST

Gene Information

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1 SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SSX2
Gene Name:   synovial sarcoma, X breakpoint 2
Aliases:   CT5.2a, HD21, HOM-MEL-40, MGC119055, MGC15364, MGC3884, SSX, SSX2B
RefSeq:   NC_000023.10 NT_011630.14
Ensembl:   ENSG00000241476
Entrez:   6757
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.270000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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