PrimePCR™ Probe Assay: MAPK10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAPK10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAPK10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040609
Assay Design:   Intron-spanning
Chromosome Location:   4:87115513-87275742question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000395169 ENST00000359221 ENST00000361569 ENST00000395166 ENST00000512017 ENST00000512564 ENST00000511167 ENST00000509464 ENST00000506773 ENST00000512689 ENST00000502302 ENST00000513186 ENST00000449047 ENST00000395161

Gene Information

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation differentiation transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2 a receptor-regulated MAP kinase scaffold protein is found to interact with and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate and inhibit the activity of this kinase which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPK10
Gene Name:   mitogen-activated protein kinase 10
Aliases:   FLJ12099, FLJ33785, JNK3, JNK3A, MGC50974, PRKM10, p493F12, p54bSAPK
RefSeq:   NC_000004.11 NG_013325.1 NT_016354.19
Ensembl:   ENSG00000109339
Entrez:   5602
Chromosome Mapping:   4q22.1-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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