PrimePCR™ Probe Assay: ATP5E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP5E, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP5E, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040732
Assay Design:   Intron-spanning
Chromosome Location:   20:57605401-57607328question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000381026 ENST00000448023 ENST00000359128 ENST00000301749 ENST00000419350 ENST00000324659 ENST00000243997 ENST00000395663 ENST00000395659 ENST00000162391 ENST00000428177 ENST00000375108

Gene Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core F1 and the membrane-spanning component Fo comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha beta gamma delta and epsilon) assembled with a stoichiometry of 3 alpha 3 beta and a single representative of the other 3. The proton channel consists of three main subunits (a b c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. Read-through transcripts that include exons from this gene are expressed from the upstream gene SLMO2.[provided by RefSeq Mar 2011]

Gene Symbol:   ATP5E
Gene Name:   ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Aliases:   ATPE, MGC104243
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000124172
Entrez:   514
Chromosome Mapping:   20q13.32

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