PrimePCR™ Probe Assay: MBOAT7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MBOAT7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MBOAT7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040738
Assay Design:   Intron-spanning
Chromosome Location:   19:54684796-54687466question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000406427 ENST00000434090 ENST00000277531 ENST00000371457 ENST00000400454 ENST00000404019 ENST00000531804 ENST00000373018 ENST00000259748 ENST00000527670 ENST00000534444 ENST00000458235 ENST00000254657 ENST00000254658 ENST00000245615 ENST00000391754 ENST00000495279 ENST00000414665 ENST00000431666 ENST00000338624 ENST00000304636 ENST00000573544 ENST00000571661 ENST00000570701 ENST00000574555 ENST00000571387 ENST00000571978 ENST00000601767 ENST00000595855 ENST00000594560 ENST00000594052 ENST

Gene Information

This gene encodes a member of the membrane-bound O-acyltransferases family of integral membrane proteins that have acyltransferase activity. The encoded protein is a lysophosphatidylinositol acyltransferase that has specificity for arachidonoyl-CoA as an acyl donor. This protein is involved in the reacylation of phospholipids as part of the phospholipid remodeling pathway known as the Land cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq Mar 2009]

Gene Symbol:   MBOAT7
Gene Name:   membrane bound O-acyltransferase domain containing 7
Aliases:   BB1, FLJ41296, LENG4, LPIAT, LRC4, MBOA7, OACT7, hMBOA-7
RefSeq:   NC_000019.9 NG_015810.1 NT_011109.16
Ensembl:   ENSG00000125505
Entrez:   79143
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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