PrimePCR™ Probe Assay: RPS9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RPS9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPS9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040885
Assay Design:   Intron-spanning
Chromosome Location:   19:54705424-54710224question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000302907 ENST00000391752 ENST00000402367 ENST00000391753 ENST00000441429 ENST00000576887 ENST00000573511 ENST00000570496 ENST00000575726 ENST00000570639 ENST00000599866 ENST00000599009 ENST00000596432 ENST00000597560 ENST00000593415 ENST00000573353 ENST00000575746 ENST00000571930 ENST00000574910 ENST00000570925 ENST00000570816

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S4P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq Jul 2008]

Gene Symbol:   RPS9
Gene Name:   ribosomal protein S9
Aliases:   S9
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000170889
Entrez:   6203
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.860000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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