PrimePCR™ Probe Assay: GABBR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GABBR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GABBR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041063
Assay Design:   Intron-spanning
Chromosome Location:   6:29591220-29595294question
Amplicon Length:   170
Splice Variants Targeted:   ENST00000563997 ENST00000566027 ENST00000552399 ENST00000383541 ENST00000383537 ENST00000383542 ENST00000383543 ENST00000548767 ENST00000414980 ENST00000434660 ENST00000423604 ENST00000438094 ENST00000311734 ENST00000383639 ENST00000383638 ENST00000383636 ENST00000383637 ENST00000551423 ENST00000323180 ENST00000418153 ENST00000257821 ENST00000312106 ENST00000449163 ENST00000439457 ENST00000452300 ENST00000419674 ENST00000546913 ENST00000550428 ENST00000547410 ENST00000400588 ENST00000448754 ENST

Gene Information

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors to produce fast synaptic inhibition and metabotropic [GABA(B)] receptors to produce slow prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis epilepsy and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq Jun 2009]

Gene Symbol:   GABBR1
Gene Name:   gamma-aminobutyric acid (GABA) B receptor, 1
Aliases:   FLJ92613, GABABR1, GABBR1-3, GB1, GPRC3A, dJ271M21.1.1, dJ271M21.1.2
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204681
Entrez:   2550
Chromosome Mapping:   6p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.470000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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