PrimePCR™ Probe Assay: ARHGAP8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ARHGAP8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARHGAP8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041157
Assay Design:   Intron-spanning
Chromosome Location:   22:45182408-45197976question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000361473 ENST00000352766 ENST00000515632 ENST00000389774 ENST00000396119 ENST00000336963 ENST00000356099 ENST00000412433 ENST00000517296 ENST00000389773

Gene Information

This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active (GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomain protein that functions to promote Erk activation and cell motility. Alternative splicing results in multiple transcript variants. Read-through transcripts from the upstream proline rich 5 renal (PRR5) gene into this gene also exist which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq Nov 2010]

Gene Symbol:   ARHGAP8
Gene Name:   Rho GTPase activating protein 8
Aliases:   BPGAP1, FLJ20185, PP610
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000241484
Entrez:   23779
Chromosome Mapping:   22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.100000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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