PrimePCR™ Probe Assay: ASAH2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041288
Assay Design:   Intron-spanning
Chromosome Location:   10:51995776-52003038question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000395526 ENST00000329428 ENST00000447815

Gene Information

Ceramidases (EC 3.5.1.23) such as ASAH2 catalyze hydrolysis of the N-acyl linkage of ceramide a second messenger in a variety of cellular events to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al. 2001 [PubMed 11328816]).[supplied by OMIM Mar 2008]

Gene Symbol:   ASAH2
Gene Name:   N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Aliases:   BCDase, HNAC1, MGC129777, NCDase
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000188611
Entrez:   56624
Chromosome Mapping:   10q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.910000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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