PrimePCR™ Probe Assay: IL32, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL32, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL32, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041315
Assay Design:   Intron-spanning
Chromosome Location:   16:3118181-3119061question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000325568 ENST00000534507 ENST00000531965 ENST00000396887 ENST00000526464 ENST00000440815 ENST00000529550 ENST00000551122 ENST00000525643 ENST00000548807 ENST00000528163 ENST00000530890 ENST00000444393 ENST00000533097 ENST00000008180 ENST00000396890 ENST00000525228 ENST00000548652 ENST00000525377 ENST00000530538 ENST00000549213 ENST00000552936 ENST00000548476 ENST00000552664 ENST00000552356 ENST00000551513 ENST00000382213

Gene Information

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site 3 potential N-myristoylation sites multiple putative phosphorylation sites and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   IL32
Gene Name:   interleukin 32
Aliases:   IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000008517
Entrez:   9235
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.260000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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