PrimePCR™ lncRNA® Probe Assay:GDNF-AS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0061081

List Price:    $254.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0138321
Assay Design:   exonic
Chromosome Location:   chr5:37874111-37874259question
Amplicon Length:   119
Refseq Transcripts Amplified:   XM_017008916,XM_017008918,XM_017008917,NM_001278094,NR_103441,NR_103442
Splice Variants Targeted:   ENST00000514532 ENST00000510986 ENST00000503382
LNCipedia Splice Variants Targeted:   GDNF-AS1:4 GDNF-AS1:2 GDNF-AS1:7 GDNF-AS1:3 GDNF-AS1:6

Gene Information

Not Available

Gene Symbol:   GDNF-AS1
Gene Name:   GDNF antisense RNA 1 (head to head) [Source:HGNC Symbol;Acc:HGNC:43592]
Aliases:   GDNFOS
RefSeq:   Not Available
Ensembl:   ENSG00000248587
LNCipedia:   GDNF-AS1
Entrez:   100861519
Chromosome Mapping:   5p13.2
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.480000
Efficiency 2

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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