PrimePCR™ lncRNA® Probe Assay:APOB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0103974

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: APOB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: APOB, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0181214
Assay Design:   exonic
Chromosome Location:   chr2:21007432-21007570question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000616098 ENST00000233242
LNCipedia Splice Variants Targeted:   lnc-C2orf43-8:1

Gene Information

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA) resulting in the creation of a stop codon and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia normotriglyceridemic hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective apoB diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq Jul 2008]

Gene Symbol:   APOB
Gene Name:   apolipoprotein B (including Ag(x) antigen)
Aliases:   FLDB, LDLCQ4
RefSeq:   NC_000002.11 NG_011793.1 NT_022184.15
Ensembl:   ENSG00000084674
LNCipedia:   lnc-C2orf43-8
Entrez:   338
Chromosome Mapping:   2p24-p23
Gene Strand:   Not Available

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