PrimePCR™ lncRNA® Probe Assay:PCA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0104820. May amplify unspliced transcripts of an antisense gene.

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: PCA3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: PCA3, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0182060
Assay Design:   exonic
Chromosome Location:   chr9:76786870-76786964question
Amplicon Length:   65
Refseq Transcripts Amplified:   NR_132312,NR_015342,NR_132313
Splice Variants Targeted:   ENST00000412654
LNCipedia Splice Variants Targeted:   PCA3:2 PCA3:1

Gene Information

Not Available

Gene Symbol:   PCA3
Gene Name:   prostate cancer associated 3 (non-protein coding) [Source:HGNC Symbol;Acc:HGNC:8637]
Aliases:   DD3, NCRNA00019, PCAT3
RefSeq:   Not Available
Ensembl:   ENSG00000225937
LNCipedia:   PCA3
Entrez:   50652
Chromosome Mapping:   9q21.2
Gene Strand:   Forward strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.950001
Efficiency 2.01

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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