PrimePCR™ lncRNA® Probe Assay:DDX21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0133418

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: DDX21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: DDX21, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0210658
Assay Design:   exonic
Chromosome Location:   chr10:68959828-68959950question
Amplicon Length:   93
Refseq Transcripts Amplified:   NM_004728,XM_011540336,NM_001256910,XM_017016910
Splice Variants Targeted:   ENST00000354185 ENST00000620315
LNCipedia Splice Variants Targeted:   lnc-DDX21-1:1

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA folds single-stranded RNA and may play important roles in ribosomal RNA biogenesis RNA editing RNA transport and general transcription. [provided by RefSeq Jul 2008]

Gene Symbol:   DDX21
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Aliases:   DKFZp686F21172, GUA, GURDB, RH-II/GU, RH-II/GuA
RefSeq:   NC_000010.10 NG_017061.1 NT_030059.13
Ensembl:   ENSG00000165732
LNCipedia:   lnc-DDX21-1
Entrez:   9188
Chromosome Mapping:   10q21
Gene Strand:   Not Available

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