PrimePCR™ lncRNA® Probe Assay:XIST, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0134689

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: XIST, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: XIST, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0211929
Assay Design:   exonic
Chromosome Location:   chrX:73829230-73831170question
Amplicon Length:   77
Refseq Transcripts Amplified:   NR_001564.2
Splice Variants Targeted:   ENST00000602863 ENST00000445814 ENST00000602587 ENST00000429829 ENST00000602495
LNCipedia Splice Variants Targeted:   XIST:9 XIST:10 XIST:15 XIST:1 XIST:3 XIST:5 XIST:11 XIST:7 XIST:8 XIST:4 XIST:2 XIST:17 XIST:13 XIST:16 XIST:19 XIST:18 XIST:12 XIST:6 XIST:14

Gene Information

X inactive specific transcript (non-protein coding)

Gene Symbol:   XIST
Gene Name:   X inactive specific transcript (non-protein coding)
RefSeq:   NR_001564
Ensembl:   ENSG00000229807
LNCipedia:   XIST
Entrez:   7503
Chromosome Mapping:   chrX:73040485-73072588
Gene Strand:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.990002
Efficiency 1.98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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