PrimePCR™ lncRNA® Probe Assay:HIF1A-AS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0134884. May amplify unspliced transcripts of an antisense gene.

List Price:    $268.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: HIF1A-AS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: HIF1A-AS2, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0212124
Assay Design:   exonic
Chromosome Location:   chr14:61748348-61748474question
Amplicon Length:   97
LNCipedia Splice Variants Targeted:   HIF1A-AS2:1 HIF1A-AS2:2

Gene Information

Not Available

Gene Symbol:   HIF1A-AS2
Gene Name:   Not Available
Aliases:   3'aHIF-1A, aHIF
RefSeq:   Not Available
LNCipedia:   HIF1A-AS2
Chromosome Mapping:   Not Available
Gene Strand:   Reverse strand

The below validation information is for the Primer Pair only  

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.500000
Efficiency 1.94

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