PrimePCR™ lncRNA® Probe Assay:SRA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0135401

List Price:    $254.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: SRA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: SRA1, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0212641
Assay Design:   exonic
Chromosome Location:   chr5:140550671-140550787question
Amplicon Length:   87
Refseq Transcripts Amplified:   NM_001035235.3,NM_001253764.1,NR_045586.1,NR_045587.1
Splice Variants Targeted:   ENST00000602657 ENST00000523259 ENST00000602775 ENST00000520427 ENST00000336283 ENST00000602875
LNCipedia Splice Variants Targeted:   lnc-APBB3-1:3

Gene Information

Both long non-coding and protein-coding RNAs are transcribed from this gene and they represent alternatively spliced transcript variants. This gene was initially defined as a non-coding RNA which is a coactivator for several nuclear receptors (NRs) and is associated with breast cancer. It has now been found that this gene is involved in the regulation of many NR and non-NR activities including metabolism adipogenesis and chromatin organization. The long non-coding RNA transcripts interact with a variety of proteins including the protein encoded by this gene. The encoded protein acts as a transcriptional repressor by binding to the non-coding RNA. [provided by RefSeq Mar 2012]

Gene Symbol:   SRA1
Gene Name:   steroid receptor RNA activator 1
Aliases:   MGC87674, SRA, SRAP, STRAA1, pp7684
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000213523
LNCipedia:   lnc-APBB3-1
Entrez:   10011
Chromosome Mapping:   5q31.3
Gene Strand:   Not Available

The below validation information is for the Primer Pair only  

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.490002
Efficiency 1.99

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