PrimePCR™ lncRNA® Probe Assay:ZFAS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info: Same primer pair as used in SYBR assay qhsaLID0070468



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PrimePCR™ IncRNA PreAmp for Probe Assay: ZFAS1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ IncRNA Template for Probe Assay: ZFAS1, Human
Reaction: 4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		lncRNA Expression
Unique Assay ID:		qhsaLIP0147708
Assay Design:		intron_spanning
Chromosome Location:		chr20:49279171-49280560question
Amplicon Length:		84
Refseq Transcripts Amplified:		NR_003604,NR_036658,NR_003605,NR_036659
Splice Variants Targeted:		ENST00000623640 ENST00000371743 ENST00000428008 ENST00000458653 ENST00000450535 ENST00000620594 ENST00000441722 ENST00000326677
LNCipedia Splice Variants Targeted:		ZFAS1:17 ZFAS1:13 ZFAS1:4 ZFAS1:10 ZFAS1:9 ZFAS1:5 ZFAS1:8 ZFAS1:2 ZFAS1:16 ZFAS1:15 ZFAS1:1 ZFAS1:11 ZFAS1:12 ZFAS1:6

Gene Information

Not Available

Gene Symbol:		ZFAS1
Gene Name:		ZNFX1 antisense RNA 1 [Source:HGNC Symbol;Acc:HGNC:33101]
Aliases:		C20orf199, HSUP1, HSUP2, NCRNA00275, ZNFX1-AS1
RefSeq:		Not Available
Ensembl:		ENSG00000177410
LNCipedia:		ZFAS1
Entrez:		441951
Chromosome Mapping:		20q13.13
Gene Strand:		Forward strand

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	36.320000
Efficiency	1.95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number	Description	Download
10039761	PrimePCR™ Assays Quick Guide, Ver B	Click to download
10042030	PrimePCR™ PreAmp Assay Quick Guide, Rev A	Click to download
10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
3226	PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays	Click to download
10000147102	PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol	Click to download