PrimePCR™ lncRNA® Probe Assay:MSLN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLID0103158

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: MSLN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: MSLN, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLIP0180398
Assay Design:   intron_spanning
Chromosome Location:   chr16:767399-768425question
Amplicon Length:   88
Refseq Transcripts Amplified:   XM_017022857,XM_011522348,XM_017022858,NM_005823,NM_001177355,XM_005255034,NM_013404,XM_017022854,XM_017022853,XM_017022856,XM_017022855
Splice Variants Targeted:   ENST00000545450 ENST00000563941 ENST00000566269 ENST00000561896 ENST00000620831 ENST00000382862 ENST00000566549
LNCipedia Splice Variants Targeted:   lnc-MSLN-1:1

Gene Information

This gene encodes a precursor protein that is cleaved into two products megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq Apr 2010]

Gene Symbol:   MSLN
Gene Name:   mesothelin
Aliases:   MPF, SMRP
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000102854
LNCipedia:   lnc-MSLN-1
Entrez:   10232
Chromosome Mapping:   16p13.3
Gene Strand:   Not Available

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