PrimePCR™ Probe Assay: Cldn13, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cldn13, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cldn13, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0028082
Assay Design:   Exonic
Chromosome Location:   5:134914989-134915088question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000008987

Gene Information

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a developmentally expressed renal tight junction protein. This gene is expressed in the cecum colon liver and kidney of mice but is not identified in rat tissues. Humans and chimpanzees lack this gene. [provided by RefSeq Aug 2010]

Gene Symbol:   Cldn13
Gene Name:   Claudin 13
Aliases:   2500004B14Rik
RefSeq:   NC_000071.6 NT_039314.8
Ensembl:   ENSMUSG00000008843
Entrez:   57255
Chromosome Mapping:   5 G2|5

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