PrimePCR™ Probe Assay: Cntf, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cntf, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cntf, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0031997
Assay Design:   Exonic
Chromosome Location:   19:12765374-12765485question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000112933

Gene Information

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq Aug 2008]

Gene Symbol:   Cntf
Gene Name:   Ciliary neurotrophic factor
Aliases:   AI429687
RefSeq:   NC_000085.6 NT_039687.8
Ensembl:   ENSMUSG00000079415
Entrez:   12803
Chromosome Mapping:   19 A|19 7.0 cM

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