PrimePCR™ Probe Assay: AC091683.2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AC091683.2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: AC091683.2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0034468
Assay Design:   Exonic
Chromosome Location:   13:49945192-49945303question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000112243 ENSMUST00000060208 ENSMUST00000106296 ENSMUST00000144153 ENSMUST00000092382 ENSMUST00000046835 ENSMUST00000143650 ENSMUST00000167242 ENSMUST00000022696 ENSMUST00000178715 ENSMUST00000097453 ENSMUST00000111117 ENSMUST00000093166 ENSMUST00000093165 ENSMUST00000165599 ENSMUST00000066175 ENSMUST00000148713 ENSMUST00000098614 ENSMUST00000106513 ENSMUST00000002145

Gene Information

Description Not Available

Gene Symbol:   AC091683.2
Gene Name:   Not available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000094886
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.250000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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