PrimePCR™ Probe Assay: Rnf44, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Rnf44, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rnf44, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0038579
Assay Design:   Exonic
Chromosome Location:   13:54683123-54683218question
Amplicon Length:   66
Splice Variants Targeted:   ENSMUST00000125871 ENSMUST00000037422 ENSMUST00000134862 ENSMUST00000150806 ENSMUST00000128257 ENSMUST00000126717 ENSMUST00000129881 ENSMUST00000177950

Gene Information

Description Not Available

Gene Symbol:   Rnf44
Gene Name:   Ring finger protein 44
Aliases:   AI854545, mKIAA1100
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000034928
Entrez:   105239
Chromosome Mapping:   13 B1|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.030000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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