PrimePCR™ Probe Assay: Sphk2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Sphk2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Sphk2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042188
Assay Design:   exonic
Chromosome Location:   7:45710419-45710524question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000107737 ENSMUST00000107738 ENSMUST00000072836

Gene Information

This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate which is involved in cell differentiation motility and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells the encoded protein is necessary for influx of calcium protein kinase C activation and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2010]

Gene Symbol:   Sphk2
Gene Name:   Sphingosine kinase 2
Aliases:   C76851
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000057342
Entrez:   56632
Chromosome Mapping:   7 B4|7

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