PrimePCR™ Probe Assay: Mrpl47, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Mrpl47, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Mrpl47, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042799
Assay Design:   exonic
Chromosome Location:   3:32727571-32727675question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000043966

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BRG1/brm-associated factor 53A (also known as BAF complex 53 kDa subunit protein A in humans) in a tail-to-tail orientation. [provided by RefSeq Jul 2008]

Gene Symbol:   Mrpl47
Gene Name:   Mitochondrial ribosomal protein L47
Aliases:   4833424P18Rik, CGI-204, ENSMUSG00000051761, Gm9859, L47mt, MRP-L47, MTF/L47, NCM1
RefSeq:   NC_000069.6 NT_162143.4
Ensembl:   ENSMUSG00000037531
Entrez:   74600
Chromosome Mapping:   3|3 B

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