PrimePCR™ Probe Assay: Coro1c, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: Coro1c, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Coro1c, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043003
Assay Design:   exonic
Chromosome Location:   5:113881958-113882078question
Amplicon Length:   91
Splice Variants Targeted:   ENSMUST00000116371 ENSMUST00000072031 ENSMUST00000121584 ENSMUST00000095849 ENSMUST00000117441 ENSMUST00000018485 ENSMUST00000168347 ENSMUST00000176824 ENSMUST00000110741 ENSMUST00000039064 ENSMUST00000123874 ENSMUST00000008893 ENSMUST00000031891 ENSMUST00000143278 ENSMUST00000095974 ENSMUST00000033804 ENSMUST00000140901 ENSMUST00000004646 ENSMUST00000163264 ENSMUST00000168399 ENSMUST00000107561 ENSMUST00000107348 ENSMUST00000092973 ENSMUST00000112777 ENSMUST00000116259 ENSMUST00000020173 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Coro1c
Gene Name:   Coronin, actin binding protein 1C
Aliases:   AL022675, AW455561, AW548837
RefSeq:   NC_000071.6 NT_078458.7
Ensembl:   ENSMUSG00000004530
Entrez:   23790
Chromosome Mapping:   5 F|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.470000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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