PrimePCR™ Probe Assay: Endog, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Endog, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Endog, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043016
Assay Design:   exonic
Chromosome Location:   2:30173833-30173970question
Amplicon Length:   108
Splice Variants Targeted:   ENSMUST00000081541 ENSMUST00000107698 ENSMUST00000052258 ENSMUST00000031594 ENSMUST00000147399 ENSMUST00000167086 ENSMUST00000045110 ENSMUST00000038677 ENSMUST00000066905 ENSMUST00000026975 ENSMUST00000170517 ENSMUST00000037504 ENSMUST00000015481 ENSMUST00000100220 ENSMUST00000179795

Gene Information

Description Not Available

Gene Symbol:   Endog
Gene Name:   Endonuclease G
Aliases:   Not Available
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000015337
Entrez:   13804
Chromosome Mapping:   2 B|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 38.990000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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