PrimePCR™ Probe Assay: Nrn1l, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Nrn1l, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nrn1l, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043157
Assay Design:   exonic
Chromosome Location:   8:105894792-105894977question
Amplicon Length:   156
Splice Variants Targeted:   ENSMUST00000119026 ENSMUST00000060303 ENSMUST00000111416 ENSMUST00000003061 ENSMUST00000155244 ENSMUST00000066723 ENSMUST00000060167 ENSMUST00000118920 ENSMUST00000117177 ENSMUST00000121369 ENSMUST00000036742 ENSMUST00000130367 ENSMUST00000053434 ENSMUST00000179968 ENSMUST00000144386 ENSMUST00000153799 ENSMUST00000111373 ENSMUST00000111375 ENSMUST00000111369 ENSMUST00000111370 ENSMUST00000111376 ENSMUST00000099723 ENSMUST00000077941 ENSMUST00000111381 ENSMUST00000111372 ENSMUST00000066420 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Nrn1l
Gene Name:   Neuritin 1-like
Aliases:   Cpg15-2, G630049C14Rik
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000044287
Entrez:   234700
Chromosome Mapping:   8 D3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.300000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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