PrimePCR™ Probe Assay: Rpl11, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best specificity

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rpl11, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rpl11, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043288
Assay Design:   exonic
Chromosome Location:   4:136052567-136052657question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000162812 ENSMUST00000161273 ENSMUST00000161938 ENSMUST00000086123 ENSMUST00000022386 ENSMUST00000125113 ENSMUST00000026698 ENSMUST00000024958 ENSMUST00000039926 ENSMUST00000037646 ENSMUST00000057037 ENSMUST00000079733 ENSMUST00000150948 ENSMUST00000059886 ENSMUST00000051377 ENSMUST00000058844 ENSMUST00000077093 ENSMUST00000023722 ENSMUST00000169721 ENSMUST00000156290 ENSMUST00000040111 ENSMUST00000171075 ENSMUST00000108372 ENSMUST00000166852 ENSMUST00000035083 ENSMUST00000178048 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rpl11
Gene Name:   Ribosomal protein L11
Aliases:   2010203J19Rik
RefSeq:   NC_000070.6 NT_187033.1
Ensembl:   ENSMUSG00000059291
Entrez:   67025
Chromosome Mapping:   4 D3|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.680000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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