PrimePCR™ Probe Assay: Sec23b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best coverage

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0044019
Assay Design:   exonic
Chromosome Location:   2:144559235-144559377question
Amplicon Length:   113
Splice Variants Targeted:   ENSMUST00000143573 ENSMUST00000028916 ENSMUST00000155258 ENSMUST00000155876 ENSMUST00000149697

Gene Information

Description Not Available

Gene Symbol:   Sec23b
Gene Name:   SEC23B (S. cerevisiae)
Aliases:   Not Available
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027429
Entrez:   27054
Chromosome Mapping:   2|2 H1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 40.410000
Efficiency 85

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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