PrimePCR™ Probe Assay: Acadvl, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Acadvl, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Acadvl, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0052534
Assay Design:   exonic
Chromosome Location:   11:70013466-70014263question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000102574 ENSMUST00000018718

Gene Information

This gene encodes a homodimeric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C16- and C20-acylCoA and localizes to the inner mitochondrial membrane (unlike related acyl-CoA dehydrogenases). In mice deficiency of this gene can cause ventricular arrhythmias as well as fasting and cold intolerance. [provided by RefSeq Nov 2012]

Gene Symbol:   Acadvl
Gene Name:   Acyl-Coenzyme A dehydrogenase, very long chain
Aliases:   vlcad
RefSeq:   NC_000077.6 NT_096135.6 NT_187026.1
Ensembl:   ENSMUSG00000018574
Entrez:   11370
Chromosome Mapping:   11 B2-B5|11 38.0 cM

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