PrimePCR™ Probe Assay: Acadvl, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Acadvl, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Acadvl, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0052534
Assay Design:   exonic
Chromosome Location:   11:70013466-70014263question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000102574 ENSMUST00000018718

Gene Information

This gene encodes a homodimeric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C16- and C20-acylCoA and localizes to the inner mitochondrial membrane (unlike related acyl-CoA dehydrogenases). In mice deficiency of this gene can cause ventricular arrhythmias as well as fasting and cold intolerance. [provided by RefSeq Nov 2012]

Gene Symbol:   Acadvl
Gene Name:   Acyl-Coenzyme A dehydrogenase, very long chain
Aliases:   vlcad
RefSeq:   NC_000077.6 NT_096135.6 NT_187026.1
Ensembl:   ENSMUSG00000018574
Entrez:   11370
Chromosome Mapping:   11 B2-B5|11 38.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.300000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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