PrimePCR™ Probe Assay: Apob, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Apob, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Apob, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0052567
Assay Design:   exonic
Chromosome Location:   12:8012361-8012506question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000037811 ENSMUST00000037520 ENSMUST00000171239

Gene Information

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human which are synthesized exclusively in the gut and liver respectively the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA) resulting in the creation of a stop codon and early translation termination. [provided by RefSeq Jul 2008]

Gene Symbol:   Apob
Gene Name:   Apolipoprotein B
Aliases:   AI315052, apob-100, apob-48
RefSeq:   NC_000078.6 NT_039548.8
Ensembl:   ENSMUSG00000020609
Entrez:   238055
Chromosome Mapping:   12 A1.1|12 2.0 cM

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