PrimePCR™ Probe Assay: Nek8, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Nek8, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nek8, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0052922
Assay Design:   exonic
Chromosome Location:   11:78172949-78173076question
Amplicon Length:   98
Splice Variants Targeted:   ENSMUST00000017549 ENSMUST00000148154

Gene Information

This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis gene A) which controls mitotic signaling in Aspergillus nidulans. [provided by RefSeq Jul 2008]

Gene Symbol:   Nek8
Gene Name:   NIMA (never in mitosis gene a)-related expressed kinase 8
Aliases:   4632401F23Rik, jck
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000017405
Entrez:   140859
Chromosome Mapping:   11 B5|11 44.0 cM

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