PrimePCR™ Probe Assay: Snrnp200, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Snrnp200, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Snrnp200, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053109
Assay Design:   exonic
Chromosome Location:   2:127230026-127230219question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000103220

Gene Information

On February 19 2002 this locus was switched from human to mouse. The source accession Z70200.1 is almost identical to the mouse BAC clone AC074224 and it matches the mouse cDNA accession BC011390 as well. The human gene is LocusID 23020. [provided by RefSeq Jul 2008]

Gene Symbol:   Snrnp200
Gene Name:   Small nuclear ribonucleoprotein 200 (U5)
Aliases:   A330064G03Rik, Ascc3l1, BC011390, HELIC2, U5-200-KD, U5-200KD
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000003660
Entrez:   320632
Chromosome Mapping:   2 F1|2 62.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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