PrimePCR™ Probe Assay: Srsf1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Srsf1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Srsf1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053118
Assay Design:   exonic
Chromosome Location:   11:88050037-88050160question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000139129 ENSMUST00000079866 ENSMUST00000107920

Gene Information

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2010]

Gene Symbol:   Srsf1
Gene Name:   Serine/arginine-rich splicing factor 1
Aliases:   1110054N12Rik, 5730507C05Rik, 6330415C05Rik, AI482334, AW491331, Asf, Sf2, Sfrs1
RefSeq:   NC_000077.6 NT_096135.6 NT_187003.1
Ensembl:   ENSMUSG00000018379
Entrez:   110809
Chromosome Mapping:   11 C|11 49.0 cM

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